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分子生物学 影印版PDF|Epub|txt|kindle电子书版本网盘下载
- P.C.Turner等著 著
- 出版社： 北京：科学出版社
- ISBN：7030073053
- 出版时间：1999
- 标注页数：307页
- 文件大小：13MB
- 文件页数：318页
- 主题词：
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图书目录
Section A-Cells and macromolecules1
A1 Cellular classification1
A2 Subcellular organelles4
A3 Macromolecules7
A4 Large macromolecular assemblies11
Section B-Protein structure15
B1 Amino acids15
B2 Protein structure and function18
B3 Protein analysis25
Section C-Properties of nucleic acids31
C1 Nucleic acid structure31
C2 Chemical and physical properties of nucleic acids36
C3 Spectroscopic and thermal properties of nucleic acids40
C4 DNA supercoiling43
Section D-Prokaryotic and eukaryotic chromosome structure47
D1 Prokaryotic chromosome structure47
D2 Chromatin structure49
D3 Eukaryotic chromosome structure54
D4 Genome complexity59
Section E-DNA replication63
E1 DNA replication:an overview63
E2 Bacterial DNA replication68
E3 Eukaryotic DNA replication72
Section F-DNA damage,repair and recombination77
F1 Mutagenesis77
F2 DNA damage81
F3 DNA repair84
F4 Recombination87
Section G-Gene manipulation91
G1 DNA cloning:an overview91
G2 Preparation of plasmid DNA96
G3 Restriction enzymes and electrophoresis99
G4 Ligation,transformation and analysis of recombinants104
Section H-Cloning vectors111
H1 Design of plasmid vectors111
H2 Bacteriophage vectors115
H3 Cosmids and YACs120
H4 Eukaryotic vectors124
Section I-Gene libraries and screening129
I1 Genomic libraries129
I2 cDNA libraries132
I3 Screening procedures137
Section J-Analysis and uses of cloned DNA141
J1 Characterization of clones141
J2 Nucleic acid sequencing146
J3 Polymerase chain reaction150
J4 Organization of cloned genes154
J5 Mutagenesis of cloned genes158
J6 Applications of cloning162
Section K-Transcription in prokaryotes165
K1 Basic principles of transcription165
K2 Escherichia coli RNA polymerase168
K3 The E.coliσ70 promoter170
K4 Transcription initiation,elongation and termination173
Section L-Regulation of transcription in prokaryotes179
L1 The lac operon179
L2 The trp operon183
L3 Transcriptional regulation by alternativeσfactors187
Section M-Transcription in eukaryotes191
M1 The three RNA polymerases:characterizationand function191
M2 RNA Pol Ⅰgenes:the ribosomal repeat193
M3 RNA Pol Ⅲ genes:5S and tRNA transcription197
M4 RNA Pol Ⅱ genes:promoters and enhancers201
M5 General transcription factors and RNA Pol Ⅱ initiation203
Section N-Regulation of transcription in eukaryotes207
N1 Eukaryotic transcription factors207
N2 Examples of transcriptional regulation213
Section O-RNA processing and RNPs219
O1 rRNA processing and ribosomes219
O2 tRNA processing,RNase P and ribozymes225
O3 mRNA processing,hnRNPs and snRNPs228
O4 Alternative mRNA processing234
Section P-The genetic code and tRNA237
P1 The genetic code237
P2 tRNA structure and function241
Section Q-Protein synthesis247
Q1 Aspects of protein synthesis247
Q2 Mechanism of protein synthesis251
Q3 Initiation in eukaryotes257
Q4 Translational control and post-translational events261
Section R-Bacteriophages and eukaryotic viruses265
R1 Introduction to viruses265
R2 Bacteriophages269
R3 DNA viruses274
R4 RNA viruses278
Section S-Tumor viruses and oncogenesb283
S1 Oncogenes found in tumor viruses283
S2 Categories of oncogenes287
S3 Tumor suppressor genes290
Further reading295
Index299